Case Studies
Allergen Reduction Study by Indoor Biotechnologies
Background
To test the ability of Betterair’s Enviro-Biotics® to reduce allergens in a scientific manner, BetterAir contacted Indoor Biotechnologies1, the leader in allergen detection worldwide. Indoor Biotechnologies tested Betterair’s Enviro-Biotics® with two ubiquitous indoor allergens:
- Dust Mite allergen Der p2 (one of the most clinically relevant allergen worldwide2,3)
- Dog allergen Can f1 (a major allergen present in dog dander extract4,5)
They incubated Enviro- Biotics® with each of the allergens for an 8-days period, and let the Enviro-Biotics® to do their work. The trial was carried out at Indoor Biotechnologies facility. Indoor Biotechnologies quantified the concentration of the allergens over time with their professional allergen analysis technology- MARIA®6. This report summarizes the preliminary experiment results.
Objective
To understand the efficacy of Enviro-Biotics® in degrading various indoor allergens under controlled experimental conditions (preliminary experiment).
Rationale
Better Air has a patented bacterial system that degrades the allergens in an indoor environment, by using them as a nutrient.
Methods
Indoor Biotechnologies used standard laboratory tubes as the controled environment. They added to each tube a small portion of our Enviro-Biotics® (only 100-200 bacteria as a starting point) and a measured portion of allergen in a scale of thousands of nanograms of each allergen (Der p1: Catalog No. NA-DP2-1, Can f1: Catalog No. NA-CF1-1). The tubes were incubated in a room temperature for 8 days, and samples were taken for bacterial concentration (using a classic microbiology method) and allergen concentration (using the INDOOR MARIA® assay, method used: MARIA SOP-001 ver.1.3).
Results and Discussion
Figures 1 and 3 show that the bacterial growth of the Enviro-Biotics® was 135 and 368 fold higher at day 8 with derp2 and Can f1 respectively.
As demonstrated in figures 2 and 4, the Enviro-Biotics® consume the allergens and decrease the allergen concentration dramatically. In Fig. 2, the Der p2 concentration was below the detectable limit at day 8.
A single Enviro- Biotics® probiotic application has decreased the allergen concentration by 85% and 80% in the allergens Dog-Can f 1 and Dust mite Der p2 respectively within 8 days.
To our knowledge, this is the first controlled experiment which demonstrates allergen consumption by microbes.
The Interpretations of Indoor Biotechnologies (we didn’t touch!)
- Since allergens were the only source of nitrogen present for the bacteria to grow, our observations support BetterAir’s view that these bacteria can grow with allergen as a source of nitrogen.
- The difference in the rate of growth of bacteria with Der p2 and Can f1 correlates well with the rate of allergen decrease.
Conclusion
Enviro-Biotics® can grow and prosper by using allergens as a nutrient source, hence dramatically decrease the concentration of allergens and reduce the side effects of ubiquitous indoor allergens on the residents.
References
- Indoor Biotechnologies website, https://inbio.com/
- Calderón, Moisés A., et al. "Respiratory allergy caused by house dust mites: what do we really know?." Journal of Allergy and Clinical Immunology 136.1 (2015): 38-48.
- Cao, Tuo, et al. "High-level expression and purification of the major house dust mite allergen Der p 2 in Escherichia coli." Protein expression and purification 121 (2016): 97-102.
- Konieczny, A., et al. "The major dog allergens, Can f 1 and Can f 2, are salivary lipocalin proteins: cloning and immunological characterization of the recombinant forms." Immunology 92.4 (1997): 577-586.
- Arbes Jr, Samuel J., et al. "Dog allergen (Can f 1) and cat allergen (Fel d 1) in US homes: results from the National Survey of Lead and Allergens in Housing." Journal of allergy and clinical immunology 114.1 (2004): 111-117.
- MARIA® by Indoor Biotechnoligies, https://inbio.com/maria-service-partners
Background:
To test the Betterair’s Enviro-Biotics ability to reduce allergens in a typical home setting. Betterair contacted EMSL Analytical, Inc. a leading microbial testing laboratory to analyize microbial samples before and after the introduction of Betterair’s Enviro-Biotics to ascertain the their efficacy to reduce common household allergens.
Sampling: First sample was taken in a family room. Second sample taken one month later in the same location.
Discovery: High level of Dust Mite Allergen (fl), Low level of Dust Mite Allergen (pl) and Moderate level of Cat Allergen.
Trial: A four week testing period of the BetterAir's probiotic purifier was conducted after initial sampling.
Results: Low level of Dust Mite Allergen (pl). All other Allergens were not detected.
Sampling: First sample taken in Master Bedroom. Second sample taken one month later in the same location.
Discovery: Low level of Dust Mite Allergen (fl), Low level of Dust Mite Allergen (pl) and Moderate level of Cockroach Allergen.
Trial: A four week testing period of the BetterAir's probiotic purifier was conducted after initial sampling.
Results: Low level of Dust Mite Allergen (fl). All other Allergens were not detected
Let customers speak for us
I’m not gonna stop now. I have been using better air Biotica800 for two years now. My family has not been sick with Covid and the few times my kids did get sick, they recovered quickly. It’s a bonus. An extra layer of protection.
So far, so good. A bit complicated but I'm learning as I've gone along. With no guidance or help with the first units I purchased, your input and a newer unit has allowed me to understand how they work. The new units and instructions are an improvement over what I first received.
When first diagnosed with CIRS, I bought the machine from BETTERAIR. Faithfully used in my bedroom after having my home fogged to kill all mold spores. Didn't think I needed any longer, but then after a couple of years, had inadvertently moved a friends furniture in my home without thinking this friend had mold. Therefore, I was getting sick from breathing in the spores. Finally, I remembered the machines I had since my Mother had passed away and inherited hers so I ordered a fresh supply Of BA-008 refill cartridges. I could feel the difference after a week of usage.
My only regret is that my Mother's machine keeps stopping and I have to manually continue to turn the machine on and giggle it to work.
My order arrived on time and was received in excellent condition.
When I started getting headaches after spending a couple hours a day in one room of my house, I took everything out of the closet and found what appeared to be mold on the floor and baseboard. My husband took out the baseboard and carpet and sprayed the area with clove oil. I put the biotica in the room and waited a week or so for it to kill the mold. The biotica made the air smell fresh. And when I started spending my mornings in the room afterwards, NO HEADACHES! Thank you for making a product that cleans the air!
Hi should've bought the bigger one for travel but this one will work just fine. I like having the spray bottle - easier to take on trips.
Although these are pretty expensive, we find that they help immensely with our allergies. Having our allergies flare up, we checked the Biotica800 to find that the cartridge was empty. We ordered them and installed them after their quick arrival. Our allergies subsided quickly. We're convinced it helps.
I find getting refill cartridges difficult. Your website does not come up on my computer
Ever since I have started using my Biotica system my breathing has been better and I feel great! Thanks
Clears the air nicely.
This is our 2nd unit - we bought our first from Amazon. We liked it so much we bought a 2nd. It's impossible to prove but our allergies have been greatly reduced since using it. Within a few days, we noticed that we're not blowing our noses and sneezing quite so often. However - all allergy related benefits are greatly reduced/lost once you go outside or open the window.
Every-so-often it lets out a puff/stream of mist. We put the first by the a/c returns for the best circulation in the area that treated the largest area of our home. Even then we saw benefits. The 2nd we bought directly from BetterAir is for the bedrooms.
Our 14 year old cat appears to be feeling better as well - she's more alert and playful. Our daughter visits weekly with her 13 year old Yorkie who suffers for a few years now from Valley Fever. He is improving as well - gaining all his pre-Valley Fever weight and is more active. Again - we can't prove any of these claims.
I have not seen a reduction in dust. I was hoping that it would magically disappear (joke), so ... I dusted. That was just yesterday and soon it will be "window opening season" and I suspect we'll be back to sneezing and blowing our noses. But - at least I got a break from my allergy medication and still have kleenex!
Love the Better air purifier. It eliminated stale orders and our facility does not smell like a dank warehouse. - Long Beach Brewing Company
